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Journal: International Journal of Molecular Sciences
Article Title: Transcriptome Analysis of Mesenchymal Progenitor Cells Revealed Molecular Insights into Metabolic Dysfunction and Inflammation in Polycystic Ovary Syndrome
doi: 10.3390/ijms25147948
Figure Lengend Snippet: Characterization of induced pluripotent stem cells (iPSCs) and iPSC-derived mesenchymal progenitor cells (iPSCMs). ( a ) Alkaline phosphatase staining showing alkaline phosphatase activity in iPSCs (blue). ( b ) Immunostaining for the pluripotency markers NANOG (red) and TRA-1-60 (green) in iPSCs. NANOG was expressed in the nuclei, while TRA-1-60 was expressed on the cell surface. Nuclei were stained with Hoechst 33,342 (blue). ( c ) Hematoxylin and eosin (H&E) staining of teratomas derived from iPSCs. The markers show the sites of lineage-specific morphological characteristics, including neuroepithelial rosette (NR) for ectoderm, chondrocyte (C) for mesoderm, and intestinal or gut-like epithelium (I/E) for endoderm. ( d ) Bright-field image showing the fibroblast-like spindle morphology of iPSCMs. ( e ) Oil red O staining of lipid droplets in adipocytes derived from iPSCMs. ( f ) Alcian blue staining for acidic polysaccharides in chondrocytes derived from iPSCMs. ( g ) Alizarin Red S staining of calcium nodules in osteoblasts derived from iPSCMs. Scale bars, 100 mm.
Article Snippet: This experiment involved the use of primary antibodies against NANOG (Cell Signaling, Danvers, MA, USA) and
Techniques: Derivative Assay, Staining, Activity Assay, Immunostaining